STUDENT RESEARCH PROJECTS

Jackson Laboratory

1998 to Present

Spring 2009

• Audrey Hendley (ABIO 499) Reduction of HIV-1 vif activity using hammerhead ribozymes (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2009, Columbia, SC)

• Christine Gross (ABIO 499) Reduction of HIV-1 vif activity using siRNA

• Lindsey Padgett (ABIO 499) Optimizing viral titer using an anti-HIV retroviral vector (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2009, Columbia, SC)

• Megan Cooper (ABIO 499) Analysis of vif5154 hammerhead ribozyme activity (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2009, Columbia, SC)

• Angela Arthur (ABIO 299) Cloning the tBid and Bax pro-apoptotic genes

Fall 2008

• Audrey Hendley (ABIO 399) A comparison of the activity of anti-HIV-1 vif ribozymes

• Christine Gross (ABIO 399) Design and cloning anti-vif siRNAS

• Lindsey Padgett (ABIO 399) Cloning anti-HIV ribozymes into a retroviral vector

• Megan Cooper (ABIO 399) Design and cloning anti-HIV ribozymes

• Angela Arthur (ABIO 299) Design and cloning of anti-HIV ribozymes

Spring 2008

• Mandy Gerolstein (ABIO 499) Expression of anti-HIV ribozymes in a tissue culture model (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2008, Clemson University, Clemson, SC)

• Rebecca Myers (ABIO 499) Design and cloning a hammerhead ribozyme to HIV nef

• Theresa Ramos (ABIO 499) Optimizing the titer of an anti-HIV retroviral vector

• Audrey Hendley (ABIO 399) Cloning anti-vif hammerhead ribozymes into a retroviral vector (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2008, Clemson University, Clemson, SC)

• Lindsey Padgett (ABIO 399) Design and cloning of a hammerhead ribozyme to the HIV LTR (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2008, Clemson University, Clemson, SC)

Fall 2007

• Mandy Gerolstein (ABIO 399) Retrovirus-mediated delivery of anti-HIV ribozymes

• Audrey Hendley (ABIO 399) Preparation of vif ribozyme templates for in vitro transcription

• Lindsey Padgett (ABIO 399) Design and cloning of a hammerhead ribozyme to the HIV LTR

• Theresa Ramos (ABIO 399) Cloning an anti-HIV -1 hammerhead ribozyme

Spring 2007

• Brooke Harrison (ABIO 499) Cloning anti-HIV LTR ribozymes into a retroviral vector (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2007, Midlands Technical College, Columbia, SC)

• Carrie Fritz-French (ABIO 499) Analysis of anti-Tat ribozyme activity using a luciferase assay (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2007, Midlands Technical College, Columbia, SC)

• Mindy McDonald (ABIO 499) Design and cloning of a ribozyme to the HIV-1 LTR (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2007, Midlands Technical College, Columbia, SC)

• Audrey Hendley (ABIO 299) Synthesis of T7 DNA templates for in vitro transcription

• Mandy Gerolstein (ABIO 299) Synthesis of T7 Vpu DNA templates for in vitro transcription

• Theresa Ramos (ABIO 299) Cloning of an anti-HIV-1 hammerhead ribozyme

Fall 2006

• Lesley Duffie (ABIO 499) Design and cloning of an anti-HIV ribozyme

• Brooke Harrison (ABIO 399) Cloning anti-HIV LTR ribozymes into a retroviral vector

• Carrie Fritz-French (ABIO 399) Analysis of anti-Tat ribozyme activity using a luciferase assay

• Mindy McDonald (ABIO 399) Cloning anti-HIV LTR ribozymes

• Nancy Jones (ABIO 399) Cloning of an anti-HIV vpu ribozyme

• Audrey Hendley (ABIO 299) Design and cloning of an anti-nef hammerhead ribozyme

• Mandy Gerolstein (ABIO 299) Design and cloning of an anti-HIV-1 hammerhead ribozyme

Spring 2006

• Connie Arthur (ABIO 499) Retrovirus-mediated delivery of anti-HIV ribozymes (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2006, University of South Carolina, Columbia, SC)

• Kalyn Estep (ABIO 499) Cloning of an anti-Tat ribozyme into a RNA Pol I cassette

• Lauren Hollingsworth (ABIO 499) Design and cloning of an anti-HIV-1 rev ribozyme

• Zach Wilson (ABIO 499) Characterization of a novel retroviral vector, pLNPolIX (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2006, University of South Carolina, Columbia, SC)

• Lesley Duffie (ABIO 399) Design and cloning of an anti-HIV-1 hammerhead ribozyme

• Brooke Harrison (ABIO 299) Design and cloning of an anti-HI LTR ribozyme

• Carrie Fritz-French (ABIO 299) Design and cloning of an anti-HIV nef ribozyme

• Garrett Webster (ABIO 299) Design and cloning of an anti-HIV LTR ribozyme

• Mindy McDonald (ABIO 299) Design and cloning of an anti-HIV LTR non-catalytic ribozyme

• Nancy Tabb Jones (ABIO 299) Ribozyme analysis techniques

Fall 2005

• Connie Arthur (ABIO 399) - Retrovirus-mediated delivery of anti-HIV ribozymes

• Lesley Duffie (ABIO 399) - Design and cloning of hammerhead ribozymes targeted to HIV-1 nef

• Lauren Hollingsworth (ABIO 399) - Cloning of an anti-HIV ribozyme into a eukaryotic plasmid

• Zach Wilson (ABIO 399) - Characterization of a novel retroviral vector, pLNPolIX

• Brooke Harrison (ABIO 299) - Ribozyme cloning into a eukaryotic expression vector

• Mindy McDonald (ABIO 299) - Design and cloning of a hammerhead ribozyme targeted to the HIV-1 LTR

Spring 2005

• Katie Anderson (ABIO 499) - Design and cloning of a hammerhead ribozyme targeted to HIV-1 Vif (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2004, Winthrop University, Rock Hill, SC)

• Carol Journey (ABIO 499) - Characterization of pCMV-TatFLAG expression in HeLa cells

• Jaina Patel (ABIO 499) - Design and cloning of an anti-HIV-1 rev hammerhead ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2004, Winthrop University, Rock Hill, SC)

• Bianca West (ABIO 499) - Design and cloning of a hammerhead ribozyme targeted to HIV-1 rev

• Connie Arthur (ABIO 399) - Design and cloning of a hammerhead ribozyme targeted to NL43 HIV-1 Vpu mRNA (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2004, Winthrop University, Rock Hill, SC)

• Lesley Duffie (ABIO 399) - Design and cloning of an anti-HIV-1 hammerhead ribozyme

• Zach Wilson (ABIO 399) - Cloning of the murine RNA Polymerase I expression cassette into a retroviral vector

• Brook Harrison (ABIO 299) - Design and cloning of an anti-HIV-1 hammerhead ribozyme targeted to the viral LTR

• Mindy McDonald (ABIO 299) - Design and cloning of an anti-HIV-1 hammerhead ribozyme targeted to the viral LTR

Fall 2004

• Shelley Blackmon (ABIO 499) - Design and cloning of a hammerhead ribozyme targeted to HIV-1 rev

• Erin Walker (ABIO 499) - Cloning of anti-HIV-1 nef ribozymes

• Katie Anderson (ABIO 399) - Design and cloning of ribozymes targeted to HIV-1 vif mRNA

• Carol Journey (ABIO 399) - Characterization of the expression vector, pCMV-tatFLAG

• Jaina Patel (ABIO 399) - Design and cloning of ribozymes targeted to HIV-1 rev

• Connie Arthur (ABIO 299) - Characterization of pCMV-VifFLAG expression

• Lesley Duffie (ABIO 299) - Cloning of hammerhead ribozymes targeted to HIV-1 nef

• Zach Wilson (ABIO 299) - Construction of an HIV-1 Rev/FLAG expression vector

Spring 2004

• Jackie Davis (ABIO 499) - Cloning of HIV-1 tat expression vectors (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

• Vanessa Guy (ABIO 499) - In vitro testing of hammerhead ribozymes targeted to HIV-1 tat RNA (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

• Rebecca Napier (ABIO 499) - Design and cloning of an anti-HIV-1 vif hammerhead ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

• Natalie Surzenko (ABIO 499) - Design and cloning of a RNA Pol I ribozyme expression vector (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

• Jackie Tilsner (ABIO 499) - Testing of tat expression vectors in a eukaryotic cell system

• Carol Journey (ABIO 399) -Structural analysis of HIV-1 tat RNA

• Erin Walker (ABIO 399) - Design and cloning of an anti-HIV-1 nef hammerhead ribozyme

• Zack Wilson - Design and cloning of an anti-HIV-1 rev hammerhead ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

Fall 2003

• Jackie Davis (ABIO 399) - Testing of hammerhead ribozyme expression in an RNA Pol I cassette

• Vanessa Guy (ABIO 399) - In vitro testing of hammerhead ribozymes targeted to HIV-1 tat RNA

• Carol Journey (ABIO 399) - Structural analysis of HIV-1 tat RNA

• Rebecca Napier (ABIO 399) - Design and cloning of an anti-HIV-1 vif hammerhead ribozyme

• Natalie Surzenko (ABIO 399) - Design and cloning of an anti-HIV-1 tat hammerhead ribozyme

• Bianca West (ABIO 299) -  Design and cloning of an anti-HIV-1 rev hammerhead ribozyme

Spring 2003

• Leigh Ryan (ABIO 499) - Design and cloning of Tat 5877 ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2003, Clemson University, Clemson, SC)

• Maggie Shoup (ABIO 499) - The cloning of hammerhead ribozyme for analysis as anti-HIV gene therapy agents: Tat 5883 (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2003, Clemson University, Clemson, SC)

• Pam Wall (ABIO 499) - Analysis of anti-tat ribozyme catalytic activity in an in vitro assay (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2003, Clemson University, Clemson, SC)

• Carol Journey (ABIO 299) - Design and cloning of the non-catalytic ribozyme Tat 5919

• OTHER STUDENTS NOT ENROLLED IN RESEARCH COURSES: Jackie Davis and Chris Perez

Fall 2002

• Leigh Ryan (ABIO 399) - Design and synthesis of an anti-tat hammerhead ribozyme: Tat 5883

• Pam Wall (ABIO 399) - Design, synthesis, and cloning of anti-tat hammerhead ribozymes: Tat 5840

• Carol Journey (ABIO 299) - Design and synthesis of an anti-tat hammerhead ribozyme: Tat 5919

Spring 2002

• Cindy Hollingshead (ABIO 499) - Use of site directed mutagenesis to analyze the function of an anti-HIV tat ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2002, University of South Carolina Aiken, Aiken, SC)

• Khai Le (ABIO 499) - Sequence analysis of genes in the bph cluster of LB400

• Jimmy Ridgeway (ABIO 499) - Development of a T-helper assay for analysis of HIV-1 Tat

• Pam Wall (ABIO 399) - Dihydrofolate Reductase (DHFR) as a selectable marker for gene therapy

Fall 2001

• Jennifer Ray (ABIO 499) - Cloning and analysis of genes in the bph cluster of LB400

• Faith Zimmerman (ABIO 499) - Indentification of the Rab11b vesicle

• OTHER STUDENTS NOT ENROLLED IN RESEARCH COURSES: Cindy Hollingshead

Spring 2001

• Jamie Holmes (ABIO 499) - Dihyrofolate Reductase (DHRF) as a selectable marker

• Shaun Opperman (ABIO 499) - Analysis of human beta-globin levels in transduced MEL cells

• OTHER STUDENTS NOT ENROLLED IN RESEARCH COURSES: Faith Zimmerman

Fall 2000

• Michael McNeill (ABIO 499) - Characterization of the use of dihydrofolate reductase (DHFR) as a marker for gene therapy in murine leukemia cells

• April Mixon (ABIO 499) - Generation of high titer ecotropic retroviral producer cells that express the human beta-globin gene (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2000, Coastal Carolina University, Conway, SC)

• William Perella (ABIO 499) - The use of anti-HIV-1 ribozymes to reduce tat expression in mammalian cells

• Amber Thacker (ABIO 399) - Optimization of electroporation characteristics for NIH-3T3 cells and their derivatives 

Spring 2000

• Briana Childers (ABIO 499) - Characterization of the use of dihydrofolate reductase (DHFR) as a selectable marker for gene therapy in murine leukemia cells

• Mindy Herrin (ABIO 499) - Characterization of beta-globin gene expression in murine erythroleukemia cells

• Matthew Swift (ABIO 499) - Global Learning and Observations to Benefit the Environment (GLOBE) and its implementation into a fourth grade science curriculum

• Leigh Stone (ABIO 199) - The use of gene therapy in the treatment of sickle cell disease

Fall 1999

NO STUDENT RESEARCHERS

Summer 1999

·         Shelly Cole (ABIO 499) - Characterization of plasmid DNA required for the production of HIV pseudotyped virus

Spring 1999

• Kathryn Burkhart (ABIO 499) - The African Dust Hypothesis: Isolation of fungal DNA from African dust samples using a lyticase protocol

• Jeff Dowdy (ABIO 499) - Relationship of African dust to coral disease: Extraction and identification of fungal DNA

• Michele Glendenning (ABIO 499) - The use of ribozymes to reduce HIV-1 tat expression in mammalian cell

Fall 1998

• Shelly Cole (ABIO 399) - Retroviral pseudotyping

• Kathryn Burkhart (ABIO 399) - Fungal ecology of tropical oceans: Sequence analysis of 18S ribosomal DNA

• Jeff Dowdy (ABIO 399) - Fungal aeromycology: RAPD (Randomly Amplified Polymorphic DNA) analysis of fungal DNA

Summer 1998

• Rhonda Foster (ABIO 499) - Identification of fungal isolates obtained from air samples at St. John's Bay, US Virgin Islands

• Nong Viangsmooth (ABIO 499) - Living with AIDS: An education program in Thailand and its effect on the AIDS rate in the Northern Region

Spring 1998

• Grant Bledsoe (ABIO 499) - Isolation and RFLP (Randomly Amplified Polymorphic DNA) analysis of bacterial DNA from a possible sea urchin pathogen  

• Joyce Bledsoe (ABIO 499) - RAPD (Randomly Amplified Polymorphic DNA) analysis of a possible sea urchin pathogen