Jackson's Laboratory Protocols

 

Molecular biology | Tissue culture | Reagents

 

MOLECULAR BIOLOGY PROTOCOLS

 

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Agarose Gel Electrophoresis

Minigel

Other gels (midi and maxi)

Minigel

Other gels (midi and maxi)

Bacterial Techniques:

Growth of Transformed Bacteria

Chemical transformation

Transformation by Electorporation

Growth in Transformed bacteria

Chemical transformation

Transformation by Electorporation

Genomic DNA Isolation:

From Blood

From mammalian Cells

From Blood

From mammalian Cells

In Vitro Transcription

 

 

Polymerase Chain Reaction

PCR, General

PCR of Genomic DNA

PCR of Plasmid DNA

PCR, General

PCR of Genomic DNA

PCR of Plasmid DNA

Preparation of Plasmid DNA:

Cesium Chloride Prep

Miniprep

Maxiprep (Qiagen)

Cesium Chloride Prep

Miniprep

Maxiprep (Qiagen)

Primers and Probes

Oligonucleotide Purification

 

Oligonucleotide Purification

 

Restriction Enzyme Digests

General

General

SDS-PAGE

 

 

Western Blot

 

 

 

 

 

REAGENTS

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TISSUE CULTURE PROTOCOLS

 

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Cell Culture Techniques, Basic:

Freezing and Thawing Cells

Hemacytometer (cell counting)

Freezing and Thawing Cells

Hemacytometer (cell counting)

 

Cell Culture Techniques, Advanced:

Lymphocyte separation from whole blood

Lymphocyte separation from whole blood

Retroviral Techniques:

Generation of Retroviral Particles

Retroviral Transduction

Generation of Retroviral Particles

Retroviral Transduction

Transfection Techniques:

Calcium-Phosphate Precipitation

Electroporation

Calcium-Phosphate Precipitation

Electroporation

 X-gal Staining of Eukaryotic Cells

 

 

 

 

 

 

 

 

 

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