Student examining a specimen in a microscope

Research Projects

Biological, Environmental, and Earth Sciences

JACKSON LAB UNDERGRADUATE RESEARCH PROJECTS

Fall 2015

Natalie Arthur (299) Analysis expression of a luciferase/GFP fusion gene in a retroviral vector

Rebecca Beaudry (199) Introduction to research in molecular biology

Kirstyn Denney (199) Introduction to research in molecular biology

Erin McLaughlin (299) Developing a HIV dependent bicistronic retroviral vector

Alyssa Smith (199) Introduction to research in molecular biology

Austin Worden (399) Cloning and analysis of an anti-HIV siRNA against Tat

Emily Webb (399)

Spring 2015 

Brittany Adams (299) Developing a transposon-based vector to express anti-HIV genes.  Awards:  Honorable mention, USCA Research Day (poster presentation).

Jennifer Deily (499) Characterization of a self-inactivating retroviral vector.  Awards: First place, USC Discovery Day (STEM B poster presentation) 

Erin McLaughlin (299) Developing a retroviral vector to express anti-HIV genes.

Natalie Arthur (199) Synthesis and cloning of a luciferase/GFP fusion gene.

Kirstyn Denney (199) Introduction to research in molecular biology.

Austin Worden (299) Design and cloning of anti-HIV reagents.

Emily Webb (399) Analysis of anti-HIV siRNA activity.  Awards"  Second place, USC Discovery Day (Biology & Biomedical Sciences A poster presentation).

Fall 2014

Jennifer Deily (399) Design and cloning of anti-HIV reagents.

Grayson Smith (399) Cloning an anti-HIV tat siRNA into a retroviral vector.

Natalie Arthur (199) Design and cloning of anti-HIV reagents.

Nicholas Hall (199) Design and cloning of anti-HIV reagents.

Erin McLaughlin (199) Developing a retroviral vector to express anti-HIV genes. 

Emily Webb (199) Analysis of anti-HIV ribozyme and siRNA reagents. 

Spring 2014

Claudia Fulmer (499) The use of pro-apoptotic genes to induce cell death in cells expressing HIV-1 tat. Awards: Silver metal, USCA Research Day (oral presentation); Sigma Xi/SCAS/Dr. Richard Petit Award for Outstanding Research, 2014 South Carolina Academy of Science meeting, Charleston, SC (oral presentation); Second place, USC Discovery Day (Biology and Biomedical Sciences II poster session) 

Crystal Ryan (499) Design and cloning an anti-tat hammerhead ribozyme. Awards: First place, USC Discovery Day (Biology and Biomedical Sciences II poster session)

Emily Webb (299) Analysis of anti-HIV ribozyme and siRNA reagents. Awards: Gold Metal, USCA Research Day (oral presentation).

Nicholas Hall (199) Design and cloning of anti-HIV reagents.

Erin McLaughlin (199) Design and cloning of anti-HIV reagents. Awards: Honorable Mention, USCA Research Day (poster presentation).

Fall 2013

Dylan Carpenter (499) Using the HIV-1 promoter/enhancer to induce cell death.

Claudia Fulmer (399) The use of pro-apoptotic genes to induce cell death in cells expressing HIV-1 tat.

Crystal Ryan (399) Introduction to research in molecular biology.

Grayson Smith (299) Introduction to research in molecular biology.

Emily Webb (299) Developing a DNA transposon-based gene delivery system.

Spring 2013

Emily Bush (499) Beta-galactosidase as a marker for HIV-1 tat-dependent expression.

Alex Jureka (499) Comparative study of anti-tat siRNAs. Awards: First place, USC Discovery Day (Biology and Medical Sciences poster session).

DeAnndra Pickens (499) Analysis of Sleeping Beauty insertion sites in HeLa cells

Priscilla Simon (499) Comparative study of HIV-1 tat induced apoptosis by pro-apoptotic Bax and tBid. Awards: AAAS Outstanding Female Scientist, 2013 South Carolina Academy of Science meeting, Columbia, SC (oral presentation); Second place, USC Discovery Day (Biology and Medical Sciences poster session).

Madison Sweet (499) Use of anti-vif ribozymes to inhibit HIV function.

Claudia Fulmer (299) Comparative study of HIV-1 tat induced apoptosis by pro-apoptotic Bax.

Emily Webb (299) Analysis of Sleeping Beauty insertion sites in 293Tcells.

Fall 2012

Sara Hopkins (499) Design and cloning anti-HIV siRNAs.

Emily Bush (399) Design and cloning a tat-dependent beta-galactosidase expression plasmid.

Alex Jureka (399) Comparative study of anti-tat siRNAs and ribozymes.

DeAnndra Pickens (399) Modification of the Sleeping Beauty transposon for gene delivery.

Priscilla Simon (399) Comparative study of HIV-1 tat induced apoptosis by pro-apoptotic Bax and tBid.

Madison Sweet (399) Use of anti-vif ribozymes to inhibit HIV function.

Tyler Ferguson (299) Developing a transposon-based DNA delivery system.

Chris Leaphart (299) Use of pro-apoptotic genes ti inhibit HIV replication.

Spring 2012

Sara Hopkins (299) Cloning anti-HIV siRNAs into a retroviral vector.

Alex Jureka (299) Analysis of anti-HIV tat expression using hammerhead ribozymes and siRNAs. Awards: Silver Award, USCA Research Day (poster session); SCAS President's Award for Outstanding Undergraduate Research, 2012 South Carolina Academy of Science meeting, Aiken, SC (oral presentation).

DeAnndra Pickens (299) Modification of the Sleeping Beauty transposon to express anti-HIV reagents.

Priscilla Simon (299) Inducement of apoptosis in a tat-dependent manner. Awards: Sigma Xi/SCAS Award for Outstanding Undergraduate Research, 2012 South Carolina Academy of Science meeting, Aiken, SC (oral presentation).

Madison Sweet (299) Design and cloning an anti-HIV siRNA.

Fall 2011

Erik Lunden (399) Cloning an anti-HIV siRNA into a retroviral vector.

Sara Hopkins (299) Cloning anti-HIV DNA-based genes.

Alex Jureka (299) Analysis of anti-HIV tat expression using siRNAs.

DeAnndra Pickens (299) Cloning anti-HIV DNA-based genes.

Priscilla Simon (299) Inducement of apoptosis in a tat-dependent manner.

Spring 2011

Renee Albano (499) Production of recombinant retroviruses in a transient system. Awards: First place, 2011 South Carolina Academy of Science meeting, Orangeburg, SC (oral presentation).

Angela Arthur (499) Inducement of apoptosis in a tat-dependent manner. Awards: First place, 2011 South Carolina Academy of Science meeting, Orangeburg, SC (oral presentation).

Destiny Murph (499) Cloning an anti-Hiv-1 tat hammerhead ribozyme into a retroviral vector. Silver Award, USCA Research Day (poster presentation).

Alex Jureka (299) Design and cloning an anti-HIV-1 tat siRNA. Awards: Co-winner with Priscilla Simon, 2011 South Carolina Academy of Science meeting, Orangeburg, SC (Cellular and Molecular Biology poster presentation).

Priscilla Simon (199) Design and cloning an anti-HIV-1 tat siRNA. Awards: Co-winner with Alex Jureka, 2011 South Carolina Academy of Science meeting, Orangeburg, SC (Cellular and Molecular Biology poster presentation).

FALL 2010

Renee Albino (399) Cloning an anti-HIV tat hammerhead ribozyme into a retroviral vector.

Angela Arthur (399) Inducement of apoptosis in a tat-dependent manner.

Destiny Murph (399) Cloning an anti-HIV-1 tat hammerhead ribozyme into a retroviral vector.

Alex Jureka (299) Introduction to the use of siRNA to inhibit HIV-1 replication.

Priscilla Simon (199) Introduction to the use of siRNA to inhibit HIV replication.

Spring 2010

Joshua Nesbitt (499) Cloning of an anti-HIV-1 tat hammerhead ribozyme into a retroviral vector Awards: Honorable Mention, USCA Research Day (poster session); Sigma Xi/SCAS Award for Outstanding Undergraduate Research and the American Association for the Advancement of Science Award to the Outstanding Male Undergraduate Scientist, 2010 South Carolina Academy of Science meeting, Charleston, SC (oral presentation).

Katie Rate (499) Analysis of an HIV-1 tat targeted siRNA. Awards: Gold Metal Award, USCA Research Day (poster session).

Angela Arthur (299) Apoptosis-mediated inhibition of HIV replication. Awards: Sigma Xi/SCAS Award for Outstanding Undergraduate Research and the Horace Byrne Explorers Club Award for Outstanding Frontier Science by an Undergraduate Scientist, 2010 South Carolina Academy of Science meeting, Charleston, SC (oral presentation).

Renee Albano (199) Cloning an anti-HIV-1 tat hammerhead ribozyme into a retroviral vector.

Fall 2009

Joshua Nesbitt (ABIO 399) Cloning an anti-HIV-1 tat hammerhead ribozyme into a retroviral vector.

Katie Rate (ABIO 399) The development of a retroviral vector expressing an anti-tat/rev siRNA.

Angela Arthur (ABIO 299) Cloning the EGFP gene into pPCR-Script.

 Spring 2009

Audrey Hendley (ABIO 499) Reduction of HIV-1 vif activity using hammerhead ribozymes.

Christine Gross (ABIO 499) Reduction of HIV-1 vif activity using siRNA.

Lindsey Padgett (ABIO 499) Optimizing viral titer using an anti-HIV retroviral vector. Awards: Gold Metal, USCA Research Day (poster session)

Megan Cooper (ABIO 499) Analysis of vif5154 hammerhead ribozyme activity.

Angela Arthur (ABIO 299) Cloning the tBid and Bax pro-apoptotic genes.

Fall 2008

Audrey Hendley (ABIO 399) A comparison of the activity of anti-HIV-1 vif ribozymes.

Christine Gross (ABIO 399) Design and cloning anti-vif siRNAS.

Lindsey Padgett (ABIO 399) Cloning anti-HIV ribozymes into a retroviral vector.

Megan Cooper (ABIO 399) Design and cloning anti-HIV ribozymes.

Angela Arthur (ABIO 299) Design and cloning of anti-HIV ribozymes.

Spring 2008

Mandy Gerolstein (ABIO 499) Expression of anti-HIV ribozymes in a tissue culture model.

Rebecca Myers (ABIO 499) Design and cloning a hammerhead ribozyme to HIV nef.

Theresa Ramos (ABIO 499) Optimizing the titer of an anti-HIV retroviral vector. Awards: Gold Metal, USCA Research Day (poster session).

Audrey Hendley (ABIO 399) Cloning anti-vif hammerhead ribozymes into a retroviral vector.

Lindsey Padgett (ABIO 399) Design and cloning of a hammerhead ribozyme to the HIV LTR.

Fall 2007

Mandy Gerolstein (ABIO 399) Retrovirus-mediated delivery of anti-HIV ribozymes.

Audrey Hendley (ABIO 399) Preparation of vif ribozyme templates for in vitro transcription.

Lindsey Padgett (ABIO 399) Design and cloning of a hammerhead ribozyme to the HIV LTR.

Theresa Ramos (ABIO 399) Cloning an anti-HIV -1 hammerhead ribozyme.

Spring 2007

Brooke Harrison (ABIO 499) Cloning anti-HIV LTR ribozymes into a retroviral vector (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2007, Midlands Technical College, Columbia, SC)

Carrie Fritz-French (ABIO 499) Analysis of anti-Tat ribozyme activity using a luciferase assay (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2007, Midlands Technical College, Columbia, SC)

Mindy McDonald (ABIO 499) Design and cloning of a ribozyme to the HIV-1 LTR (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2007, Midlands Technical College, Columbia, SC)

Audrey Hendley (ABIO 299) Synthesis of T7 DNA templates for in vitro transcription

Mandy Gerolstein (ABIO 299) Synthesis of T7 Vpu DNA templates for in vitro transcription

Theresa Ramos (ABIO 299) Cloning of an anti-HIV-1 hammerhead ribozyme

Fall 2006

Lesley Duffie (ABIO 499) Design and cloning of an anti-HIV ribozyme.

Brooke Harrison (ABIO 399) Cloning anti-HIV LTR ribozymes into a retroviral vector.

Carrie Fritz-French (ABIO 399) Analysis of anti-Tat ribozyme activity using a luciferase assay.

Mindy McDonald (ABIO 399) Cloning anti-HIV LTR ribozymes.

Nancy Jones (ABIO 399) Cloning of an anti-HIV vpu ribozyme.

Audrey Hendley (ABIO 299) Design and cloning of an anti-nef hammerhead ribozyme.

Mandy Gerolstein (ABIO 299) Design and cloning of an anti-HIV-1 hammerhead ribozyme.

Spring 2006

Connie Arthur (ABIO 499) Retrovirus-mediated delivery of anti-HIV ribozymes (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2006, University of South Carolina, Columbia, SC)

Kalyn Estep (ABIO 499) Cloning of an anti-Tat ribozyme into a RNA Pol I cassette.

Lauren Hollingsworth (ABIO 499) Design and cloning of an anti-HIV-1 rev ribozyme.

Zach Wilson (ABIO 499) Characterization of a novel retroviral vector, pLNPolIX (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2006, University of South Carolina, Columbia, SC).

Lesley Duffie (ABIO 399) Design and cloning of an anti-HIV-1 hammerhead ribozyme.

Brooke Harrison (ABIO 299) Design and cloning of an anti-HI LTR ribozyme.

Carrie Fritz-French (ABIO 299) Design and cloning of an anti-HIV nef ribozyme.

Garrett Webster (ABIO 299) Design and cloning of an anti-HIV LTR ribozyme

Mindy McDonald (ABIO 299) Design and cloning of an anti-HIV LTR non-catalytic ribozyme.

Nancy Tabb Jones (ABIO 299) Ribozyme analysis techniques.

Fall 2005

Connie Arthur (ABIO 399) - Retrovirus-mediated delivery of anti-HIV ribozymes.

Lesley Duffie (ABIO 399) - Design and cloning of hammerhead ribozymes targeted to HIV-1 nef.

Lauren Hollingsworth (ABIO 399) - Cloning of an anti-HIV ribozyme into a eukaryotic plasmid.

Zach Wilson (ABIO 399) - Characterization of a novel retroviral vector, pLNPolIX.

Brooke Harrison (ABIO 299) - Ribozyme cloning into a eukaryotic expression vector.

Mindy McDonald (ABIO 299) - Design and cloning of a hammerhead ribozyme targeted to the HIV-1 LTR.

Spring 2005

Katie Anderson (ABIO 499) - Design and cloning of a hammerhead ribozyme targeted to HIV-1 Vif (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2004, Winthrop University, Rock Hill, SC)

Carol Journey (ABIO 499) - Characterization of pCMV-TatFLAG expression in HeLa cells

Jaina Patel (ABIO 499) - Design and cloning of an anti-HIV-1 rev hammerhead ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2004, Winthrop University, Rock Hill, SC)

Bianca West (ABIO 499) - Design and cloning of a hammerhead ribozyme targeted to HIV-1 rev

Connie Arthur (ABIO 399) - Design and cloning of a hammerhead ribozyme targeted to NL43 HIV-1 Vpu mRNA (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, March 2004, Winthrop University, Rock Hill, SC)

Lesley Duffie (ABIO 399) - Design and cloning of an anti-HIV-1 hammerhead ribozyme

Zach Wilson (ABIO 399) - Cloning of the murine RNA Polymerase I expression cassette into a retroviral vector

Brook Harrison (ABIO 299) - Design and cloning of an anti-HIV-1 hammerhead ribozyme targeted to the viral LTR

Mindy McDonald (ABIO 299) - Design and cloning of an anti-HIV-1 hammerhead ribozyme targeted to the viral LTR

Fall 2004

Shelley Blackmon (ABIO 499) - Design and cloning of a hammerhead ribozyme targeted to HIV-1 rev

Erin Walker (ABIO 499) - Cloning of anti-HIV-1 nef ribozymes

Katie Anderson (ABIO 399) - Design and cloning of ribozymes targeted to HIV-1 vif mRNA

Carol Journey (ABIO 399) - Characterization of the expression vector, pCMV-tatFLAG

Jaina Patel (ABIO 399) - Design and cloning of ribozymes targeted to HIV-1 rev

Connie Arthur (ABIO 299) - Characterization of pCMV-VifFLAG expression

Lesley Duffie (ABIO 299) - Cloning of hammerhead ribozymes targeted to HIV-1 nef

Zach Wilson (ABIO 299) - Construction of an HIV-1 Rev/FLAG expression vector

Spring 2004

Jackie Davis (ABIO 499) - Cloning of HIV-1 tat expression vectors (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

Vanessa Guy (ABIO 499) - In vitro testing of hammerhead ribozymes targeted to HIV-1 tat RNA (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

Rebecca Napier (ABIO 499) - Design and cloning of an anti-HIV-1 vif hammerhead ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

Natalie Surzenko (ABIO 499) - Design and cloning of a RNA Pol I ribozyme expression vector (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

Jackie Tilsner (ABIO 499) - Testing of tat expression vectors in a eukaryotic cell system

Carol Journey (ABIO 399) -Structural analysis of HIV-1 tat RNA

Erin Walker (ABIO 399) - Design and cloning of an anti-HIV-1 nef hammerhead ribozyme

Zack Wilson - Design and cloning of an anti-HIV-1 rev hammerhead ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2004, College of Charleston, Charleston, SC)

Fall 2003

Jackie Davis (ABIO 399) - Testing of hammerhead ribozyme expression in an RNA Pol I cassette

Vanessa Guy (ABIO 399) - In vitro testing of hammerhead ribozymes targeted to HIV-1 tat RNA

Carol Journey (ABIO 399) - Structural analysis of HIV-1 tat RNA

Rebecca Napier (ABIO 399) - Design and cloning of an anti-HIV-1 vif hammerhead ribozyme

Natalie Surzenko (ABIO 399) - Design and cloning of an anti-HIV-1 tat hammerhead ribozyme

Bianca West (ABIO 299) -  Design and cloning of an anti-HIV-1 rev hammerhead ribozyme

Spring 2003

Leigh Ryan (ABIO 499) - Design and cloning of Tat 5877 ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2003, Clemson University, Clemson, SC)

Maggie Shoup (ABIO 499) - The cloning of hammerhead ribozyme for analysis as anti-HIV gene therapy agents: Tat 5883 (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2003, Clemson University, Clemson, SC)

Pam Wall (ABIO 499) - Analysis of anti-tat ribozyme catalytic activity in an in vitro assay (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2003, Clemson University, Clemson, SC)

Carol Journey (ABIO 299) - Design and cloning of the non-catalytic ribozyme Tat 5919

OTHER STUDENTS NOT ENROLLED IN RESEARCH COURSES: Jackie Davis and Chris Perez

Fall 2002

Leigh Ryan (ABIO 399) - Design and synthesis of an anti-tat hammerhead ribozyme: Tat 5883

Pam Wall (ABIO 399) - Design, synthesis, and cloning of anti-tat hammerhead ribozymes: Tat 5840

Carol Journey (ABIO 299) - Design and synthesis of an anti-tat hammerhead ribozyme: Tat 5919

Spring 2002

Cindy Hollingshead (ABIO 499) - Use of site directed mutagenesis to analyze the function of an anti-HIV tat ribozyme (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2002, University of South Carolina Aiken, Aiken, SC)

Khai Le (ABIO 499) - Sequence analysis of genes in the bph cluster of LB400

Jimmy Ridgeway (ABIO 499) - Development of a T-helper assay for analysis of HIV-1 Tat

Pam Wall (ABIO 399) - Dihydrofolate Reductase (DHFR) as a selectable marker for gene therapy

Fall 2001

Jennifer Ray (ABIO 499) - Cloning and analysis of genes in the bph cluster of LB400

Faith Zimmerman (ABIO 499) - Indentification of the Rab11b vesicle

OTHER STUDENTS NOT ENROLLED IN RESEARCH COURSES: Cindy Hollingshead

Spring 2001

Jamie Holmes (ABIO 499) - Dihyrofolate Reductase (DHRF) as a selectable marker

Shaun Opperman (ABIO 499) - Analysis of human beta-globin levels in transduced MEL cells

OTHER STUDENTS NOT ENROLLED IN RESEARCH COURSES: Faith Zimmerman

Fall 2000

Michael McNeill (ABIO 499) - Characterization of the use of dihydrofolate reductase (DHFR) as a marker for gene therapy in murine leukemia cells

April Mixon (ABIO 499) - Generation of high titer ecotropic retroviral producer cells that express the human beta-globin gene (PRESENTED AT THE SOUTH CAROLINA ACADEMY OF SCIENCES, April 2000, Coastal Carolina University, Conway, SC)

William Perella (ABIO 499) - The use of anti-HIV-1 ribozymes to reduce tat expression in mammalian cells

Amber Thacker (ABIO 399) - Optimization of electroporation characteristics for NIH-3T3 cells and their derivatives 

Spring 2000

Briana Childers (ABIO 499) - Characterization of the use of dihydrofolate reductase (DHFR) as a selectable marker for gene therapy in murine leukemia cells

Mindy Herrin (ABIO 499) - Characterization of beta-globin gene expression in murine erythroleukemia cells

Matthew Swift (ABIO 499) - Global Learning and Observations to Benefit the Environment (GLOBE) and its implementation into a fourth grade science curriculum

Leigh Stone (ABIO 199) - The use of gene therapy in the treatment of sickle cell disease

Fall 1999

NO STUDENT RESEARCHERS

Summer 1999

Shelly Cole (ABIO 499) - Characterization of plasmid DNA required for the production of HIV pseudotyped virus

Spring 1999

Kathryn Burkhart (ABIO 499) - The African Dust Hypothesis: Isolation of fungal DNA from African dust samples using a lyticase protocol

Jeff Dowdy (ABIO 499) - Relationship of African dust to coral disease: Extraction and identification of fungal DNA

Michele Glendenning (ABIO 499) - The use of ribozymes to reduce HIV-1 tat expression in mammalian cell

Fall 1998

Shelly Cole (ABIO 399) - Retroviral pseudotyping

Kathryn Burkhart (ABIO 399) - Fungal ecology of tropical oceans: Sequence analysis of 18S ribosomal DNA

Jeff Dowdy (ABIO 399) - Fungal aeromycology: RAPD (Randomly Amplified Polymorphic DNA) analysis of fungal DNA

Summer 1998

Rhonda Foster (ABIO 499) - Identification of fungal isolates obtained from air samples at St. John's Bay, US Virgin Islands

Nong Viangsmooth (ABIO 499) - Living with AIDS: An education program in Thailand and its effect on the AIDS rate in the Northern Region

Spring 1998

Grant Bledsoe (ABIO 499) - Isolation and RFLP (Randomly Amplified Polymorphic DNA) analysis of bacterial DNA from a possible sea urchin pathogen  

Joyce Bledsoe (ABIO 499) - RAPD (Randomly Amplified Polymorphic DNA) analysis of a possible sea urchin pathogen